In this section
@article{Southall:2008:10.1101/pdb.prot5029,
author = {Southall, TD and Brand, AH},
doi = {10.1101/pdb.prot5029},
journal = {CSH Protoc},
title = {Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila.},
url = {http://dx.doi.org/10.1101/pdb.prot5029},
volume = {2008},
year = {2008}
}
TY - JOUR
AB - INTRODUCTIONThe GAL4 system is a method for ectopic gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. This protocol describes the generation of driver and reporter lines for use with the GAL4 system in Drosophila. A promoter-GAL4 fusion is constructed using a P-element transformable vector, and a GAL4-responsive target gene is created via generation of an upstream activation sequence (UAS)-reporter construct. An alternative strategy for integration using the phiC31 system is also provided. Transformant lines are generated using standard procedures for microinjection.
AU - Southall,TD
AU - Brand,AH
DO - 10.1101/pdb.prot5029
PY - 2008///
TI - Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila.
T2 - CSH Protoc
UR - http://dx.doi.org/10.1101/pdb.prot5029
UR - https://www.ncbi.nlm.nih.gov/pubmed/21356873
VL - 2008
ER -
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