In this section
@article{Spillane:2018:10.1007/978-1-4939-7474-0_5,
author = {Spillane, KM and Tolar, P},
doi = {10.1007/978-1-4939-7474-0_5},
journal = {Methods in Molecular Biology},
pages = {69--80},
title = {DNA-based probes for measuring mechanical forces in cell-cell contacts: Application to B cell antigen extraction from immune synapses},
url = {http://dx.doi.org/10.1007/978-1-4939-7474-0_5},
volume = {1707},
year = {2018}
}
TY - JOUR
AB - The production of antibodies requires the expansion and selection of high-affinity B cell clones. This process is initiated by antigen uptake through the B cell receptor (BCR), which recognizes and binds antigen displayed on the surface of an antigen-presenting cell (APC). To acquire the antigen, B cells use myosin contractility to physically pull BCR-antigen clusters from the APC membrane. These mechanical forces influence association and dissociation rates of BCR-antigen bonds, resulting in affinity-dependent acquisition of antigen by B cells. Mechanical regulation of B cell antigen acquisition from APCs remains poorly understood, although the recent development of DNA-based force sensors has enabled the measurement of mechanical forces generated in B cell-APC contacts. In this chapter, we describe a protocol to design, synthesize, and purify DNA-based force sensors to measure B cell antigen extraction forces using fluorescence microscopy.
AU - Spillane,KM
AU - Tolar,P
DO - 10.1007/978-1-4939-7474-0_5
EP - 80
PY - 2018///
SN - 1940-6029
SP - 69
TI - DNA-based probes for measuring mechanical forces in cell-cell contacts: Application to B cell antigen extraction from immune synapses
T2 - Methods in Molecular Biology
UR - http://dx.doi.org/10.1007/978-1-4939-7474-0_5
UR - https://www.ncbi.nlm.nih.gov/pubmed/29388100
VL - 1707
ER -
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