In this section
@article{Hidalgo:2022:10.1016/j.isci.2022.103879,
author = {Hidalgo, D and Martinez-Ortiz, CA and Palsson, BO and Jimenez, J and Utrilla, J},
doi = {10.1016/j.isci.2022.103879},
journal = {iScience},
title = {Regulatory perturbations of ribosome allocation in bacteria reshape the growth proteome with a trade-off in adaptation capacity},
url = {http://dx.doi.org/10.1016/j.isci.2022.103879},
volume = {25},
year = {2022}
}
TY - JOUR
AB - Bacteria regulate their cellular resource allocation to enable fast growth-adaptation to a variety of environmental niches. We studied the ribosomal allocation, growth, and expression profiles of two sets of fast-growing mutants of Escherichia coli K-12 MG1655. Mutants with only three of the seven copies of ribosomal RNA operons grew faster than the wild-type strain in minimal media and show similar phenotype to previously studied fast-growing rpoB mutants. Comparing these two different regulatory perturbations (rRNA promoters or rpoB mutations), we show how they reshape the proteome for growth with a concomitant fitness cost. The fast-growing mutants shared downregulation of hedging functions and upregulated growth functions. They showed longer diauxic shifts and reduced activity of gluconeogenic promoters during glucose-acetate shifts, suggesting reduced availability of the RNA polymerase for expressing hedging proteome. These results show that the regulation of ribosomal allocation underlies the growth/hedging phenotypes obtained from laboratory evolution experiments.
AU - Hidalgo,D
AU - Martinez-Ortiz,CA
AU - Palsson,BO
AU - Jimenez,J
AU - Utrilla,J
DO - 10.1016/j.isci.2022.103879
PY - 2022///
SN - 2589-0042
TI - Regulatory perturbations of ribosome allocation in bacteria reshape the growth proteome with a trade-off in adaptation capacity
T2 - iScience
UR - http://dx.doi.org/10.1016/j.isci.2022.103879
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000773615400010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.sciencedirect.com/science/article/pii/S2589004222001493?via%3Dihub
UR - http://hdl.handle.net/10044/1/99431
VL - 25
ER -
Interested in studying a PhD at the Department of Life Sciences? Find out more about postgraduate research opportunties.