BibTex format
@article{Nasilli:2026:10.1161/CIRCEP.125.014354,
author = {Nasilli, G and Lin, X and Swiatlowska, P and Meraviglia, V and Pérez-Hernández, M and Zhang, M and Sanchez-Alonso, JL and Bellin, M and Gorelik, J and Rothenberg, E and Casini, S and Delmar, M and Remme, CA},
doi = {10.1161/CIRCEP.125.014354},
journal = {Circ Arrhythm Electrophysiol},
title = {Decreasing Microtubule Detyrosination Improves Cardiac Mechanics and Sodium Channel Function in Arrhythmogenic Cardiomyopathy.},
url = {http://dx.doi.org/10.1161/CIRCEP.125.014354},
volume = {19},
year = {2026}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Alterations in microtubule dynamics have been shown to affect cardiomyocyte membrane stiffness and modulate ion channels, including the cardiac sodium channel. While conditions, such as heart failure and Duchenne muscular dystrophy, are associated with increased detyrosination of microtubules and reduced sodium current, a potential role for microtubule detyrosination in arrhythmogenic cardiomyopathy has not been explored. We here investigated the impact of microtubule detyrosination on membrane stiffness, cardiac sodium channel distribution, and function in mouse and human models of arrhythmogenic cardiomyopathy. METHODS: Isolated ventricular cardiomyocytes from mice with cardiomyocyte-specific, tamoxifen-activated knockout of PKP2 (plakophilin-2), as well as PKP2-c.2013delC, and isogenic control human-induced pluripotent stem cell-derived-cardiomyocytes were incubated for 2 to 4 hours with compounds known to decrease microtubule detyrosination (parthenolide, 10 µmol/L; EpoY, 20 µmol/L) or vehicle (dimethyl sulfoxide). Immunocytochemistry, mechano-scanning ion conductance microscopy, patch-clamp analysis, and stochastic optical reconstruction microscopy were performed. RESULTS: Cardiomyocyte-specific, tamoxifen-activated knockout of PKP2 mouse cardiomyocytes displayed increased microtubule detyrosination and membrane stiffness, which were both attenuated by parthenolide treatment. Parthenolide significantly increased whole-cell sodium current density in cardiomyocyte-specific, tamoxifen-activated knockout of PKP2 mouse cardiomyocytes, with macropatch measurements demonstrating that this increase occurred both at the intercalated disc and lateral membrane. Stochastic optical reconstruction microscopy analysis revealed that parthenolide increased cardiac sodium channel cluster density at the intercalated disc of cardiomyocyte-specific, tamoxifen-activated knockout of PKP2 mouse cardiomyocytes. In contrast, parthenolide had no effect on sodium c
AU - Nasilli,G
AU - Lin,X
AU - Swiatlowska,P
AU - Meraviglia,V
AU - Pérez-Hernández,M
AU - Zhang,M
AU - Sanchez-Alonso,JL
AU - Bellin,M
AU - Gorelik,J
AU - Rothenberg,E
AU - Casini,S
AU - Delmar,M
AU - Remme,CA
DO - 10.1161/CIRCEP.125.014354
PY - 2026///
TI - Decreasing Microtubule Detyrosination Improves Cardiac Mechanics and Sodium Channel Function in Arrhythmogenic Cardiomyopathy.
T2 - Circ Arrhythm Electrophysiol
UR - http://dx.doi.org/10.1161/CIRCEP.125.014354
UR - https://www.ncbi.nlm.nih.gov/pubmed/42366968
VL - 19
ER -