We provide direct independent equipment access to our collaborators.
Our instruments are available on collaborative use with a fee to help cover service contracts only. You must complete a lab induction before using the collaboration area and booking equipment for the first time. To request a lab induction or make a booking, you would need an iLab account. Once you have created an account, please contact Jessica Tam or Hamza Halane to arrange a suitable time for your induction. Refer to iLab Booking Instructions.docx if you are unsure about how to make a booking on iLab.
After you have completed the initial lab induction, additional induction and training sessions are required for the use of the cryostats, microtomes, and slide scanner. To arrange the cryostat and microtome induction, please contact Nathalie Lambie. To arrange the slide scanner training, please contact Nik Matthews with the following information:
- What tissue type you are working with.
- Which imaging mode you wish to use - brightfield or fluorescence (FL).
- For brightfield - the stains you are using.
- For FL - the markers you are using and the excitation and emission values of the markers.
- Whether you already have experience with microscopy and on the AxioScan 7.
Rainin and Eppendorf pipettes are available in the collaboration area but the user must provide any required kits, chips, and consumables including pipette tips. Please only use the equipment supplied on the shared facility benches.
It is your responsibility to keep the collaboration area tidy which includes changing the bin, recycling tip boxes, safely disposing of reagents, telling us if something is broken or not working, etc. If you need any advice (e.g. a place to put old or unused reagents, find new and dispose of old bin bags, etc) please speak to us.
List of Collaborative Access Instruments
- Invitrogen Qubit 2 - A benchtop Fluorometer used for the quantitation of DNA, RNA and protein.
- Agilent Bioanalyser – An automated electrophoresis instrument for QC assessment of sizing,integrity and quantitation of RNA and DNA.
- Applied Biosystems StepOnePlus™ Real-Time PCR System – 96 well Real-Time PCR instrument
- Eppendorf Speed Vac – The Eppendorf Concentrator faciliates fast and efficient, gently vacuum concentration of DNA, RNA, nucleotides, proteins and other liquid samples.
- 10X Genomics Chromium Controller iX – The 10X controller uses advanced microfludics to perform single cell partitioning and barcoding.
- Luna FL – The Luna FL can assess cell counts and viability without being limited by cell type orsize. It can count in both brightfield and fluorescence mode.
- LevitasBio LeviCell 1.0 – The LeviCell is used to perform label free, gentle sample enrichment to increase your sample viability and reduce dead cells and debris.
- cellenONE – A standalone cellenOne instrument allowing single cell sorting and isolation based on cell morphology and multichannel fluorescence. Using gently acoustic waves for droplet generation, cellenOne preserves outstanding cell viability and maintains proteins expression for single cell sequencing applications.
- Life Technologies Countess II – The Countess II is a compact, benchtop automated cell counter to provide accurate cell counting. It has digital imaging with autofocus and an analysis algorithm to identify, count and determine viability.
- Biorad C1000 Thermal Cycler – The C1000 Touch thermal cycler offers superior performance and a touch screen for easy programming. It has a fully adjusted heated lid and a gradient-enabled 96 deep well module.
- Leica Cryostat CM1950 Config.5EU – The cryostat for laboratories with a large number of specimens and varying specimen types.
- Leica Microtome HistoCore MULTICUT R Config. 1 – MULTICUT HistoCore AUTOCUT Microtome type Mechanical manual Semi-motorized, manual Fully motorized Personalized coarse feed wheel for improved comfort. Unique force balance system eliminates the risk of injury by an unbalanced object head.
- ZEISS Axioscan 7 – A high-performance slide scanner for Fluorescence, Brightfield and Polarization.
- MGISP-100 Automated Sample Preparation System – An automated workstation for high throughput sequencing library preparation. It processes samples in batches to increase stability of library preparation.
- Covaris E220X Focused Ultrasonicator – A sonicator that enables multi-sample preparation with scalable acoustic energy and can process a wide range of sample types and volumes.
