Spatial Genomics

The Nanostring GeoMx® Digital Spatial Profiler modified from https://nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/ 

• Resolves tissue heterogeneity & complexity of microenvironments
• Provides flexible and robust spatial multi-omic platform for analysis of FFPE and fresh frozen tissue sections
• Non-destructively profiles expression of RNA and protein from distinct tissue compartments and cell populations
• Integrates with standard histology staining
• Spatially profiles the whole transcriptome and 570+ protein targets separately or simultaneously from your choice of sample inputs – whole tissue sections, tissue microarrays (TMAs), or organoids

The Nanostring CosMx™ Single-Cell Imaging modified from https://nanostring.com/products/cosmx-spatial-molecular-imager/single-cell-imaging-overview/

• Provides high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) & fresh frozen (FF) tissue samples at cellular & subcellular resolution
• Enables rapid quantification & visualization of up to 6,000 RNA & 64 validated protein analytes

Enables deeper insights for:

• Cell atlasing
• Tissue phenotyping
• Cell-cell interactions
• Cellular processes
• Biomarker discovery

Stereo-seq by STOmics modified from https://en.stomics.tech/ 

STOmics proprietary Stereo-seq (SpaTial Enhanced REsolution Omics-sequencing) technology offers researchers a novel tool to explore spatial biology with unprecedented field-of-view, and subcellular resolution, enabling simultaneous transcriptome study and analysis at tissue, cellular, subcellular and molecular level from fresh frozen tissue.

Stereo-seq facilitates the in-situ capture of RNA information within tissue on the chip and subsequently restores cellular spatial locational data through spatial coordination barcoding (Coordinate ID, CID). This advanced approach enables the realization of spatial gene expression profiles of tissues following sequencing on the DNBSEQ platform, expediting the establishment of robust research frameworks for delving into the intricate interplay between gene expression, cell morphology, and cellular microenvironments.

Curio Seeker modified from https://curiobioscience.com/seeker/

Curio Seeker Spatial Transcriptomics Kits enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution and are compatible with your favourite eukaryotic organism. The assay plugs directly into existing sequencing workflows and requires no new specialized instrumentation or novel probe sets.

Curio Seeker tile preserves spatial information of the tissue transcriptome by capturing RNA on a monolayer of tightly packed, spatially indexed 10μm beads. Once your tissue section is placed on the Curio Seeker tile, mRNA is captured and hybridized on the spatially indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. Purified cDNA undergoes NGS library preparation and is sequenced. The resulting FASTQ files are processed by the Curio Seeker bioinformatics pipeline to create a continuous whole transcriptome spatial map of your tissue section.

Curio Trekker modified from https://curiobioscience.com/curio-trekker/

Curio Trekker integrates seamlessly with existing single-cell sequencing workflows and preserves the high molecular sensitivity of single-cell data. Curio Trekker works by tagging each nucleus within its native tissue environment with unique spatial barcodes. These spatial barcodes are read using next-generation sequencing (NGS), allowing each nucleus to be bioinformatically positioned in its spatial coordinates. The result is a spatial map with true single-cell resolution without the use of complex instrumentation, cell-type deconvolution, or cell segmentation.