We are highly trained and experienced on NGS technologies and applications. We provide professional support from experimental design and grant application stages to project timelines, costs and quotations. We welcome collaborations with members of the Imperial College London, universities within the UK and overseas, as well as commercial partners on a fee-for-service basis. Please contact us to discuss your requirements.
Key phases of your project after a consultation
The quality and quantity of your submitted DNA or RNA samples are initially checked at our facility and you will be contacted if there is any sample that appears below our expectations.
We offer a broad range of library preparation options depending on the sample and application type and source. We offer library preparation for the following applications:
- Whole-Genome Sequencing
- Exome Sequencing
- Targeted Resequencing
- Transcriptome Sequencing
- Pathogen Sequencing
- Amplicon Sequencing
- ChIP Sequencing
- Single-Cell Sequencing
Short fragment libraries that will be prepared from your samples, will be individually barcoded to be pooled if more than one sample is going to be sequenced in a single run. These individual "barcode" sequences will help to identify each sample read during the final data analysis. We combine libraries so each indexed sample is present at equimolar concentration in the pool and to result in equal distribution of reads during sequencing.
We also have automated liquid handling instruments (Agilent Bravo) in place to help with some of the large-scale projects and are happy to discuss bespoke protocols.
At the Facility, we have sequencing platforms from Illumina (NextSeq2000) and MGI (G99, G400, T7 and coming soon T1+) also in addition we can provide NovaSeq sequencing via our external collaborators at ICR and Francis Crick Institute. The different output of each of our sequencing platforms provide flexibility to meet various throughput needs. The generated data then will be processed through a demultiplexing pipeline that will sort the reads into different files according to their individual barcode sequence. The raw data will be delivered in the FASTQ files format through Globus only.
We do not offer pooling of the libraries that have not been prepared within the Facility; you should be able to provide the pool(s) of barcoded libraries for a sequencing-only request.
Bioinformatics analysis can be offered upon a consultation. We have access to dedicated high-performance computing resources at the Imperial High-Performance Computing Service and Data Centre and maintain analysis pipelines for large-scale genomic datasets from a wide range of sequencing applications.
