In this section
@article{Springael:1996,
author = {Springael, D and van, Thor JJ and Goorissen, H and Ryngaert, A and De, Baere R and van, Hauwe P and Commandeur, LC and Parsons, JR and De, Wacheter R and Mergeay, M},
journal = {Microbiology},
pages = {3282--3293},
title = {RP4::Mu3A-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway},
url = {http://mic.sgmjournals.org/cgi/reprint/142/11/3283?view=long&pmid=8969525},
volume = {142},
year = {1996}
}
TY - JOUR
AB - Chromosomal DNA fragments encoding the ability to utilize biphenyl as sole carbon source (Bph+) were mobilized by means of plasmid RP4::Mu3A from strain JB1 (tentatively identified as Burkholderia sp.) to Alcaligenes eutrophus CH34 at a frequency of 10(-3) per transferred plasmid. The mobilized DNA integrated into the recipient chromosome or was recovered as catabolic prime plasmids. Three Bph+ prime plasmids were transferred from A. eutrophus to Escherichia coli and back to A. eutrophus without modification of the phenotype. The transferred Bph+ DNA segments allowed metabolism of biphenyl, 2-, 3- and 4-chlorobiphenyl, and diphenylmethane. Genes involved in biphenyl degradation were identified on the prime plasmids by DNA-DNA hybridization and by gene cloning. Bph+ prime plasmids were transferred to Burkholderia cepacia, Pseudomonas aeruginosa, Comamonas testosteroni and A. eutrophus and the catabolic genes were expressed in those hosts. Transfer of the plasmid to the 3-chlorobenzoate-degrading bacterium Pseudomonas sp. B13 allowed the recipient to mineralize 3-chlorobiphenyl. Other catabolic prime plasmids were obtained from JB1 by selection on m-hydroxybenzoate and tyrosine as carbon sources. 16S rRNA sequence data demonstrated that the in vivo transfer of bph was achieved between bacteria belonging to two different branches of the beta-Proteobacteria.
AU - Springael,D
AU - van,Thor JJ
AU - Goorissen,H
AU - Ryngaert,A
AU - De,Baere R
AU - van,Hauwe P
AU - Commandeur,LC
AU - Parsons,JR
AU - De,Wacheter R
AU - Mergeay,M
EP - 3293
PY - 1996///
SP - 3282
TI - RP4::Mu3A-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway
T2 - Microbiology
UR - http://mic.sgmjournals.org/cgi/reprint/142/11/3283?view=long&pmid=8969525
VL - 142
ER -
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