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@article{Rutherford:1988:10.1016/0005-2728(88)90152-1,
author = {Rutherford, AW and Seibert, M and Metz, JG},
doi = {10.1016/0005-2728(88)90152-1},
journal = {Bba Bioenergetics},
pages = {171--176},
title = {Characterisation of the low-fluorescent (LF1) mutant of Scenedesmus by EPR},
url = {http://dx.doi.org/10.1016/0005-2728(88)90152-1},
volume = {932},
year = {1988}
}
TY - JOUR
AB - An EPR study of the low fluorescent mutant, LF1, of the green alga, Scenedesmus obliquus, has been performed and the following results were obtained. (1) Stable photoinduced charge separation occurs at low temperature forming the characteristic EPR signals from Q<sup>-</sup><inf>A</inf>Fe<sup>2+</sup>, the primary semiquinone-iron complex, indicating no major structural modification of the electron acceptor complex in the LF1 mutant. (2) The source of the electron in this reaction at 200 K is a component, probably a chlorophyll molecule, which gives rise to an EPR signal at g ≈ 2.0026 when photooxidized; in the wild type the S<inf>2</inf> manganese multiline EPR signal is generated under these conditions. (3) Contrary to a previous report (Biochim. Biophys. Acta 682 (1982) 106-114), Signal II is present in the dark in LF1; however, it exhibits different microwave power saturation characteristics from those of Signal II in the wild type. These results show that the LF1 mutant has a functional reaction centre which lacks the redox active manganese of the O<inf>2</inf>-evolving enzyme. In addition, as reported previously, cytochrome b-559 is in the low-potential, oxidized form in LF1, while it is largely in the high-potential, reduced form in the wild-type. This effect is thought to be only indirectly related to the 34 kDa to the 36 kDa change in D<inf>1</inf> polypeptide size, which is assumed to be responsible for the lack of Mn binding in the LF1 mutant. © 1988.
AU - Rutherford,AW
AU - Seibert,M
AU - Metz,JG
DO - 10.1016/0005-2728(88)90152-1
EP - 176
PY - 1988///
SN - 0005-2728
SP - 171
TI - Characterisation of the low-fluorescent (LF1) mutant of Scenedesmus by EPR
T2 - Bba Bioenergetics
UR - http://dx.doi.org/10.1016/0005-2728(88)90152-1
VL - 932
ER -
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