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@article{Wisden:1997:10.1042/bst025535sa,
author = {Wisden, W},
doi = {10.1042/bst025535sa},
journal = {Biochemical Society Transactions},
title = {Ligand-gated ion channel subunit partnerships: GABAA receptor α6 subunit gene inactivation inhibits δ subunit expression},
url = {http://dx.doi.org/10.1042/bst025535sa},
volume = {25},
year = {1997}
}
TY - JOUR
AB - Cerebellar granule cells express six GABA<inf>A</inf> receptor subunits abundantly (α<inf>1</inf>, α<inf>6</inf>, β<inf>2</inf>, β<inf>3</inf>, γ<inf>2</inf> and δ), and assemble a variety of pentameric receptor subtypes, with unknown subunit compositions. However, the rules guiding receptor subunit assembly are unclear. Removal of intact α<inf>6</inf> protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied (Jones et al., 1997). Exon 8 of the mouse α<inf>6</inf> -/- subunit gene was disrupted by homologous recombination. In α<inf>6</inf> -1- granule cells, the δ subunit was selectively degraded as seen by immunoprecipitation, immunocytochemistry, and immunoblot analysis with δ subunit-specific antibodies. The δ subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the δ subunit. These results provide genetic evidence for a specific association between the α<inf>6</inf> and δ subunits.
AU - Wisden,W
DO - 10.1042/bst025535sa
PY - 1997///
SN - 0300-5127
TI - Ligand-gated ion channel subunit partnerships: GABAA receptor α6 subunit gene inactivation inhibits δ subunit expression
T2 - Biochemical Society Transactions
UR - http://dx.doi.org/10.1042/bst025535sa
VL - 25
ER -
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