In this section
@article{Davis:1996:10.1007/BF00726723,
author = {Davis, DM and Birch, DJ},
doi = {10.1007/BF00726723},
journal = {J Fluoresc},
pages = {23--32},
title = {Extrinsic fluorescence probe study of human serum albumin using Nile red.},
url = {http://dx.doi.org/10.1007/BF00726723},
volume = {6},
year = {1996}
}
TY - JOUR
AB - Nile red bound to human serum albumin (HSA) shows an order of magnitude increase in the probe's fluorescence intensity. Here, we report on the fluorescence characteristics of the probe-protein complex in Trizma buffer (pH 7.1), urea, guanidine hydrochloride, and AOT/isooctane/buffer reverse micelles using both steady-state and time-resolved fluorescence techniques. With a view to illustrating the use of extrinsic probe fluorescence spectroscopy in protein research, we demonstrate that protein unfolding can be observed through measurements of the probe's time-resolved anisotropy and steady-state fluorescence spectrum. Moreover, this shows that thermal unfolding is fundamentally different from using denaturant, with respect to changes in both the nanosecond diffusional rotation of the probe at intermediate stages and in the denatured protein's structure. Also, the large Stokes shift of Nile red allows the changes in the environment of the probe-protein complex in reverse micelles of varying waterpool size to be easily identified in the steady-state fluorescence. This was not seen in earlier work exploiting the intrinsic tryptophan fluorescence of HSA and further demonstrates the complementary information that extrinsic fluorescence probe studies can offer protein science. We discuss the complex acrylamide quenching characteristics of Nile red bound to HSA in terms of the possibility of at least two binding sites for the probe and the effect of acrylamide on the probe-protein structure at very high quencher concentrations.
AU - Davis,DM
AU - Birch,DJ
DO - 10.1007/BF00726723
EP - 32
PY - 1996///
SN - 1053-0509
SP - 23
TI - Extrinsic fluorescence probe study of human serum albumin using Nile red.
T2 - J Fluoresc
UR - http://dx.doi.org/10.1007/BF00726723
UR - https://www.ncbi.nlm.nih.gov/pubmed/24226994
VL - 6
ER -
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