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@article{Zainal:2011,
author = {Zainal, N and Turnbull, C},
journal = {Asia Pacific Journal of Molecular Biology and Biotechnology},
pages = {121--130},
title = {Short communication: Efects of diferent light conditions on various CONSTANS-LIKE mutants and their response to fowering time},
volume = {19},
year = {2011}
}
TY - JOUR
AB - CONSTANS (CO) is a central component in the photoperiod pathway that is involved in promotion of fowering. In Arabidopsis, CO promotes fowering under long day conditions. CONSTANS-LIKE 1 (COL1) is one of the genes in the CO family with three conserved regions including two domains near the amino terminus known as zinc fnger regions and a CCT (CONSTANS, CO-like, and TOC1) domain near the carboxy terminus. Unexpectedly, in this study, the results show that a col1 T-DNA insertion mutant exhibited early fowering under long day and short day conditions while a COL4 transferred DNA (T-DNA) line showed early fowering under short day but not under long day conditions. An insertion in the 3'UTR of col1 was confrmed by PCR. Gene expression analysis showed that the COL1 gene was disrupted. However, through expression analysis using several primers, at least a partial COL1 mRNA was shown to be present in the col1 line. Nevertheless, whether the mRNA is functional and able to be translated to COL1 protein is still uncertain. Tis indicates that col1 is a mutant but not a complete knockout. Under light-limited condition analysis, wild type, col1 and col12 plants exhibited early fowering under high light intensity compared to low light intensity. Our results suggest that COL1 acts as a negative regulator of fowering time while COL4 only afects fowering time under short day conditions. Additionally, faster growth and fowering time in Arabidopsis under higher light intensity may be due to an increased photosynthetic rate.
AU - Zainal,N
AU - Turnbull,C
EP - 130
PY - 2011///
SN - 0128-7451
SP - 121
TI - Short communication: Efects of diferent light conditions on various CONSTANS-LIKE mutants and their response to fowering time
T2 - Asia Pacific Journal of Molecular Biology and Biotechnology
VL - 19
ER -
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