This post has been created as part of a multidisciplinary project funded by the Wellcome Trust to investigate the neuro-immune-stromal interactions in response to environmental change and how this drives allergic airway inflammation and remodelling. Specifically, this post will investigate the cellular interactions between fibroblasts, matrix and immune cells that drive airway remodelling during severe asthma. The successful candidate will use...
Job listing information
- Reference MED02199
- Date posted 11 January 2021
- Closing date 10 February 2021
This post has been created as part of a multidisciplinary project funded by the Wellcome Trust to investigate the neuro-immune-stromal interactions in response to environmental change and how this drives allergic airway inflammation and remodelling. Specifically, this post will investigate the cellular interactions between fibroblasts, matrix and immune cells that drive airway remodelling during severe asthma. The successful candidate will use a combination of in vitro cell culture techniques, mass spectrometry-based proteomics coupled with cutting edge microscopy and cell imaging techniques, on cells from asthma patients and mouse models.
The post holder will use systems well established in the group but will have the freedom to develop novel systems to investigate our hypothesis. Once appointed, the post holder will be involved in developing and driving the project as well as writing up work for presentation and publication. The ability to work independently and as part of a team, excellent communication, organisational and problem-solving skills are essential. You will be technically proficient at a high level to ensure up to date support for research (https://lloydlab.co.uk).
Duties and responsibilities
You will be:
- Using multi-parameter IHC, visualise the spatial location, cell-cell and cell-matrix interactions in lungs from mouse models and in bronchoscopy biopsies from patients with severe asthma.
- Identify and develop suitable techniques, software, equipment for the collection and analysis of data.
- To conduct murine models of allergic airways disease pathology, assess lung function, sampling of the airways and tissue processing.
- To investigate phenotypic and molecular characteristics of pulmonary cells in health and disease by flow cytometry and transcriptomic analysis.
- To conduct cytokine assays, histological analysis, immunocytochemistry, RNA extraction and real time PCR.
You will have:
- PhD in Immunology or a closely related discipline
- Knowledge or experience of Second Harmonic Generation microscopy
- Experience and/or knowledge of Proteomics Mass Spectrometry
- Experience of analysing ‘omics data
- Knowledge of public bioinformatics resources for biological data interrogation and interpretation
- Microscopy and imaging experience (Confocal, widefield, live cell imaging or Hyperion Mass Cytometry) including use of analysis software (Image J, ICY, IMARIS, Volocity)
- Home Office Personal License / in vivo experience
- Experience with Primary Cell Culture
This is a full time, fixed term contract for 2 years and you will be required to complete an online application for this post.
Candidates who have not yet been officially awarded their PhD will be appointed as a Research Assistant within the salary range £36,045 - £39,183 per annum
Should you require any further details on the role please contact: Laura Yates, Research Manager Lloyd Lab - email@example.com.
For technical issues when applying online please email firstname.lastname@example.org
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Please note that job descriptions cannot be exhaustive and the post-holder may be required to undertake other duties, which are broadly in line with the above key responsibilities.
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