Research Associate

Job summary

This post has been created as part of a multidisciplinary project funded by the Wellcome Trust to investigate the neuro-immune-stromal interactions in response to environmental change and how this drives allergic airway inflammation and remodelling. Specifically, this post will investigate the cellular interactions between fibroblasts, matrix and immune cells that drive airway remodelling during severe asthma. The successful candidate will use...

Job listing information

  • Reference MED02199
  • Date posted 11 January 2021
  • Closing date 10 February 2021

Job description

Job summary

This post has been created as part of a multidisciplinary project funded by the Wellcome Trust to investigate the neuro-immune-stromal interactions in response to environmental change and how this drives allergic airway inflammation and remodelling. Specifically, this post will investigate the cellular interactions between fibroblasts, matrix and immune cells that drive airway remodelling during severe asthma. The successful candidate will use a combination of in vitro cell culture techniques, mass spectrometry-based proteomics coupled with cutting edge microscopy and cell imaging techniques, on cells from asthma patients and mouse models.

The post holder will use systems well established in the group but will have the freedom to develop novel systems to investigate our hypothesis. Once appointed, the post holder will be involved in developing and driving the project as well as writing up work for presentation and publication. The ability to work independently and as part of a team, excellent communication, organisational and problem-solving skills are essential. You will be technically proficient at a high level to ensure up to date support for research (https://lloydlab.co.uk).

Duties and responsibilities

You will be:

  • Using multi-parameter IHC, visualise the spatial location, cell-cell and cell-matrix interactions in lungs from mouse models and in bronchoscopy biopsies from patients with severe asthma.
  • Identify and develop suitable techniques, software, equipment for the collection and analysis of data.
  • To conduct murine models of allergic airways disease pathology, assess lung function, sampling of the airways and tissue processing.
  • To investigate phenotypic and molecular characteristics of pulmonary cells in health and disease by flow cytometry and transcriptomic analysis.
  • To conduct cytokine assays, histological analysis, immunocytochemistry, RNA extraction and real time PCR.

Essential requirements

You will have:

  • PhD in Immunology or a closely related discipline
  • Knowledge or experience of Second Harmonic Generation microscopy   
  • Experience and/or knowledge of Proteomics Mass Spectrometry
  • Experience of analysing ‘omics data
  • Knowledge of public bioinformatics resources for biological data interrogation and interpretation
  • Microscopy and imaging experience (Confocal, widefield, live cell imaging or Hyperion Mass Cytometry) including use of analysis software (Image J, ICY, IMARIS, Volocity)
  • Home Office Personal License / in vivo experience
  • Experience with Primary Cell Culture

Further information

This is a full time, fixed term contract for 2 years and you will be required to complete an online application for this post.

Candidates who have not yet been officially awarded their PhD will be appointed as a Research Assistant within the salary range £36,045 - £39,183 per annum

Should you require any further details on the role please contact: Laura Yates, Research Manager Lloyd Lab -  l.yates@imperial.ac.uk.

For technical issues when applying online please email recruitment@imperial.ac.uk

 

The College is a proud signatory to the San-Francisco Declaration on Research Assessment (DORA), which means that in hiring and promotion decisions, we evaluate applicants on the quality of their work, not the journal impact factor where it is published. For more information, see https://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-evaluation/

The College believes that the use of animals in research is vital to improve human and animal health and welfare. Animals may only be used in research programmes where their use is shown to be necessary for developing new treatments and making medical advances. Imperial is committed to ensuring that, in cases where this research is deemed essential, all animals in the College’s care are treated with full respect, and that all staff involved with this work show due consideration at every level.

http://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-integrity/animal-research

Documents

About Imperial College London

Imperial College London is the UK’s only university focussed entirely on science, engineering, medicine and business and we are consistently rated in the top 10 universities in the world.

You will find our main London campus in South Kensington, with our hospital campuses located nearby in West and North London. We also have Silwood Park in Berkshire and state-of-the-art facilities in development at our major new campus in White City.

We work in a multidisciplinary and diverse community for education, research, translation and commercialisation, harnessing science and innovation to tackle the big global challenges our complex world faces.

It’s our mission to achieve enduring excellence in all that we do for the benefit of society – and we are looking for the most talented people to help us get there.

Additional information

Please note that job descriptions cannot be exhaustive and the post-holder may be required to undertake other duties, which are broadly in line with the above key responsibilities.

All Imperial employees are expected to follow the 7 principles of Imperial Expectations: 

  • Champion a positive approach to change and opportunity
  • Communicate regularly and effectively within, and across, teams
  • Consider the thoughts and expectations of others
  • Deliver positive outcomes
  • Encourage inclusive participation and eliminate discrimination
  • Develop and grow skills and expertise
  • Work in a planned and managed way 

In addition to the above, employees are required to observe and comply with all College policies and regulations.

Imperial College is committed to equality of opportunity, to eliminating discrimination and to creating an inclusive working environment. We are an Athena SWAN Silver award winner, a Stonewall Diversity Champion, a Disability Confident Employer and work in partnership with GIRES to promote respect for trans people.