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@article{Cao:2022:10.1016/j.ebiom.2022.103867,
author = {Cao, XE and Ongagna-Yhombi, SY and Wang, R and Ren, Y and Srinivasan, B and Hayden, JA and Zhao, Z and Erickson, D and Mehta, S},
doi = {10.1016/j.ebiom.2022.103867},
journal = {EBioMedicine},
title = {A diagnostic platform for rapid, simultaneous quantification of procalcitonin and C-reactive protein in human serum.},
url = {http://dx.doi.org/10.1016/j.ebiom.2022.103867},
volume = {76},
year = {2022}
}
TY - JOUR
AB - BACKGROUND: Early and accurate determination of bacterial infections as a potential cause for a patient's systemic inflammatory response is required for timely administration of appropriate treatment and antibiotic stewardship. Procalcitonin (PCT) and C-reactive protein (CRP) have both been used as biomarkers to infer bacterial infections, particularly in the context of sepsis. There is an urgent need to develop a platform for simultaneous quantification of PCT and CRP, to enable the potential use of these biomarkers at the point-of-care. METHODS: A multiplexed lateral flow assay (LFA) and a fluorescence optical reader were developed. Assay performance was validated by testing spiked antigens in the buffer, followed by a validation study comparing results with conventional assays (Roche Cobas e411 Elecsys PCT and Siemens ADVIA XPT CRP) in 25 archived remnant human serum samples. FINDINGS: A linear regression correlation of 0·97 (P < 0·01) was observed for PCT, and a correlation of 0·95 (P < 0·01) was observed for CRP using direct patient samples. We also validated our platform's ability to accurately quantify high-dose CRP in the hook effect range where excess unlabeled analytes occupy binding sites at test lines. INTERPRETATION: A fluorescence reader-based duplex LFA for simultaneous quantification of PCT and CRP was developed and successfully validated with clinical samples. The rapid, portable, and low-cost nature of the platform offers potential for differentiation of bacterial and viral infections in emergency and low-resource settings at the point-of-care. FUNDING: NIH/NIBIB Award 1R01EB021331, and Academic Venture Fund from the Atkinson Center for a Sustainable Future at Cornell University.
AU - Cao,XE
AU - Ongagna-Yhombi,SY
AU - Wang,R
AU - Ren,Y
AU - Srinivasan,B
AU - Hayden,JA
AU - Zhao,Z
AU - Erickson,D
AU - Mehta,S
DO - 10.1016/j.ebiom.2022.103867
PY - 2022///
TI - A diagnostic platform for rapid, simultaneous quantification of procalcitonin and C-reactive protein in human serum.
T2 - EBioMedicine
UR - http://dx.doi.org/10.1016/j.ebiom.2022.103867
UR - https://www.ncbi.nlm.nih.gov/pubmed/35149284
VL - 76
ER -
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