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@article{Buck:2015:10.1007/978-1-4939-2392-2_4,
author = {Buck, M and Engl, C and Joly, N and Jovanovic, G and Jovanovic, M and Lawton, E and McDonald, C and Schumacher, J and Waite, C and Zhang, N},
doi = {10.1007/978-1-4939-2392-2_4},
journal = {Methods Mol Biol},
pages = {53--79},
title = {In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.},
url = {http://dx.doi.org/10.1007/978-1-4939-2392-2_4},
volume = {1276},
year = {2015}
}

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TY  - JOUR
AB  - Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ(54)), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ(54)-dependent gene expression.
AU  - Buck,M
AU  - Engl,C
AU  - Joly,N
AU  - Jovanovic,G
AU  - Jovanovic,M
AU  - Lawton,E
AU  - McDonald,C
AU  - Schumacher,J
AU  - Waite,C
AU  - Zhang,N
DO  - 10.1007/978-1-4939-2392-2_4
EP  - 79
PY  - 2015///
SP  - 53
TI  - In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-4939-2392-2_4
UR  - https://www.ncbi.nlm.nih.gov/pubmed/25665558
VL  - 1276
ER  -