Titan Krios
The ThermoFisher Krios G3i equipped with a Gatan K3 direct detector and BioQuantum energy filter represents the facility’s flagship data collection instrument. This state-of-the-art cryo-EM system enables high-resolution single-particle analysis and tomography with exceptional stability and automation. The combination of a 300 kV field emission source, advanced energy filtering, and fast direct electron detection provides outstanding image quality, routinely achieving near-atomic resolution for challenging biological specimens.
LonCEM Krios/K3
Krios G3i specification
- ThermoFisher Krios G3i (version 3.1) Cryo-EM:
- Accelerating Voltage: 300 kV field emission gun (X-FEG)
- Energy Filter: Ametek/Gatan BioQuantum 967 GIF Quantum LS
- Detectors: Ametek/Gatan K3; Falcon 3 direct electron detectors; Ceta 16M
- Autoloader: 12-grid (cassette) or 100-grid (magazine) capacity
- Cryo Stage: High-stability, side-entry autoloader, <1 nm drift/min
- Condenser & Objective Lenses: Constant-power lenses for automated operation
- AFIS (abberation free image-beam shift) & FFI (fringe free) imaging configuration
- Phase Plate: Volta phase plate option for contrast enhancement
- Cryo-Tomography: Fully compatible with TOMO5 tomography
- Automation: EPU software for unattended data collection
- Resolution: Routine single-particle reconstructions to ~2 Å (specimen-dependent)
- Sample Handling: Cryo-ready, contamination-protected transfer system
- Throughput: Up to 20,000 micrographs/day under automated operation depending on configuration•